
ACS Clinical Guidelines
- ACS Guideline for PNH Immunophenotyping
- ACS Guideline For Lymphocyte Subset Immunophenotyping – 3rd Edition
- ACS Guideline for HLAB27 Immunophenotyping
- ACS Guideline for Clinical Flow Cytometry Laboratory Practice – 4th Ed 2025
- ACS Guideline for Haematology Oncology Immunophenotyping – 4th Ed 2025
- ACS CHECKLIST Laboratory Assessment of Haematology Oncology Immunophenotyping
- ACS CHECKLIST Assessment of Clinical Flow Cytometry Laboratory Practice
- ACS Guideline for CD34 Haematopoietic Progenitor Cell Enumeration
- ACS Guideline for Flow Cytometric Assessment of HLA Alloantibodies
- ACS Guideline for Eosin-5-Malemide testing of RBCs
- ACS Guideline for Minimal Residual Disease
- ACS Guideline for Fetomaternal Haemorrhage Testing
Background
The ACS first developed clinical guidelines in 2006 to support the design and
implementation of both malignant and non-malignant flow cytometric assays. These
consensus-based guidelines are created by subject matter experts from across
Australia and New Zealand and are regularly reviewed to remain current and
relevant to the Australian clinical cytometry community.
The guidelines outline minimum testing requirements to assist laboratories in
establishing new assays, reviewing existing ones, and serving as reference material
for assessments and audits.
Active engagement from ACS members is essential to support the adoption and
ongoing refinement of these guidelines.
For comments or expressions of interest, please contact us
ACS CLINICAL GUIDELINE COMMITTEE
Committee Chair
- Susan Wright, (TAS), Senior Scientific Officer, Royal Hobart Hospital
Members
- Neil Came, (VIC), Medical Staff Specialist, Peter MacCallum Cancer Centre
- William Sewell (NSW), Medical Staff Specialist, Garvan Institute of Med Research
- Neil McNamara, (JPN), Senior Scientist (ret)
- Michelle Burns, (NZ), Technical Lead, Canterbury Health Laboratories
- Vuong Nguyen, (VIC), Lab Manager, Peter MacCallum Cancer Centre
Contact: clinicalguidelines@cytometry.org.au
ACS CLINICAL GUIDELINE WORK GROUPS
Clinical Flow Laboratory Practice
Chair
- Neil McNamara, (JPN), Senior Scientist (ret)
Members
- Andrea Baker, (NZ), Scientist, Canterbury Health Laboratory
- Marian Fernandez, (NSW), Senior Scientist, ICPMR Westmead Hospital
- Claire Lloyd, (WA), Senior Scientific Officer, Western Diagnostic Pathology
- Kate Marson, (NZ), Technical Specialist, Labplus Auckland City Hospital
- Quynhlam Nguyen, (SA), Section Head, SA Pathology
ACS Guideline for Clinical Flow Cytometry Laboratory Practice
Haematology, Oncology, Immunophenotyping
Chair
- Neil Came, (VIC), Medical Staff Specialist, Peter MacCallum Cancer Centre
Members
- Edward Abadir (NSW), Medical Staff Specialist, Royal Prince Alfred Hospital
- Kerrie Clerici, (VIC), Lab Manager, Cell Therapy and Flow Cytometry Laboratory
- Na Kang, (QLD), Senior Scientist, Princess Alexandra Hospital
- Aruna Kodituwakka, (QLD), Medical Staff Specialist, Pathology Queensland
- Jing Liu, (VIC), Senior Scientific Officer, Northern Health Pathology
- Kate Marson, (NZ), Technical Specialist, Labplus Auckland Hospital
- Eugene Ng, (SA), Scientist, SA Pathology
- Tracey Nguyen, (NSW), Senior Scientific Officer, Liverpool Hospital
- Kerryn Stoner, (WA), Scientist (FC in Charge), PathWest
- Elizabeth Todorova, (VIC), SSO, Eastern Health
- Susan Wright, (TAS), Senior Scientific Officer, Royal Hobart Hospital
ACS Guideline for Haematology Oncology Immunophenotyping
Lymphocyte Subset Enumeration
Chair
- Paula Keating, (NZ), Senior Scientific Officer, Canterbury Health Laboratory
Members
- Monica Kemp, (WA), Scientist in Charge, QE II Immunology, Pathwest
- Athanasios Krotiris, (SA), Scientist, SA Pathology
- Jing Liu, (VIC), Senior Scientific Officer, Northern Health Pathology
- William Sewell (NSW), Medical Staff Specialist, Garvan Institute of Med Research
ACS Guideline For Lymphocyte Subset Immunophenotyping
Paroxysmal Nocturnal Haemoglobinuria
- Susan Wright, Senior Scientist Royal Hobart Hospital
- Deni Cupit, Scientist, Royal Hobart Hospital
- Tania Jobey, Senior Scientist, PathWest
- Na Kang, Senior Scientist, Princess Alexandra Hospital
- Kate Marson, Technical Specialist, Labplus Auckland City Hospital
- Eugene Ng, Scientist, SA Pathology
ACS Guideline for PNH Immunophenotyping
CD34 Enumeration
- Michelle Petrasich (chair), NZ, Senior Scientist, Auckland City Hospital
- Susan Wright, TAS, Senior Scientist, Royal Hobart Hospital
- Sandy Smith, NSW, Senior Scientist, St Vincent’s Sydney
- Vuong Nguyen, VIC, Senior Scientist, Peter MacCallum Cancer Centre
- Neil McNamara, NSW, Senior Scientist, Liverpool Sydney
ACS Guideline for CD34 Haematopoietic Progenitor Cell Enumeration
HLA Alloantibodies and HLAB27 Immunophenotyping
- Susan Wright, Senior Scientist Royal Hobart Hospital
- Quynhlam Nguyen, Head of Flow Cytometry, SA Pathology
- Tracey Nguyen, Senior Scientific Officer, Liverpool Hospital
ACS Guideline for Flow Cytometric Assessment of HLA Alloantibodies
ACS Guideline for HLAB27 Immunophenotyping
Hereditary Spherocytosis
- Janine Davies, WA, Scientist PathWest, QEII Medical Centre Perth
- Donna Cross, QLD, Senior Scientist, Path Qld, Princess Alexandra, Brisbane
ACS Guideline for Eosin-5-Maldemide Testing of Red Blood Cells
Minimal Residual Disease Testing
- Vuong Nguyen, VIC, Senior Scientist, Peter MacCallum Cancer Centre
- Neil Came, VIC, Haematologist, Peter MacCallum Cancer Centre
- Aruna Kodituwakku, SA, Immunopathologist, Royal Adelaide Hospital
- Gosia Gorniak, VIC, Senior Scientist, Alfred Hospital Melbourne
- Mary Sartor, NSW, Senior Scientist, Westmead Sydney
Haemoglobin F
- Alireza Ardjmand, NSW, Senior Scientist, NSW Health Pathology
- Michelle Burns, NZ, Technical Lead, Canterbury Health Laboratories
- Michael Gooch, QLD, Medical Scientist, QML Pathology
- Pam Stewart, NZ, Senior Scientist, Waikato DHB
- Steve Valentine, Application Specialist, Abacus Dx
Myeloma
Guideline in development – can you help? Contact us
Primary Immune Deficiencies
Guideline in development – can you help? Contact us
Myelodysplastic Syndrome
Guideline in development – can you help? Contact us
Platelet Assays
Guideline in development – can you help? Contact us
Neutrophil Function
Guideline in development – can you help? Contact us
