Cytometry Basics

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Compensation:

Dr. Mario Roederer’s personal website provides an excellent tutorial on fluorescence compensation. The tutorial includes a variety of topics including how to perform proper compensation, errors in compensation, and how to deal with autofluorescence. The website also includes a quiz.

Chromocyte: a beginners guide to flow cytometry:

The Chromocyte website provides a useful “introduction to flow cytometry” video from Innova Biosciences. The video gives a length overview of various aspects of flow cytometry.

Introductory training: :

The BD Bioscience website has a helpful introductory module, which provides an overview of flow cytometry. Topics include simple aspects such as sample types and particle sizes, as well as introductions to optics and fluorescence.

History of Flow Cytometry:

The chromocyte website links to a brief module on the historical origins of flow cytometry.

Compensation Webinar:

A webinar from Miltenyi that explains the fundamentals of fluorescence compensation.

Biocompare:

The Biocompare website allows you to search for a wide variety of antibodies from a range of companies. This is especially useful in panel design, as you can very quickly determine the range of conjugates that may be available for each of the antibodies in your panel. The Biocompare search covers a large number of common vendors for flow cytometry reagents.

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