SOPs and Protocols

These SOP's and Protocols are a made available by the ACS to assist Research members in their day-to-day duties and to encourage and assist research cytometrists to further develop their knowledge and skill base.

As the ACS is run by members for the benefit of its members most of this content has been generated by ACS membres themselves, if you have something you'd like to share please let us know. Submission can be made through the link below  

Below are some examples of the most recent examples of Research Resources . If you'd like more Research Protocols or Standard Operating Procedures please follow the links in the main menu.

Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria :

 

Cell division is an essential cellular process that requires an array of known and unknown proteins for its spatial and temporal regulation. Here we develop a novel, high-throughput screening method for the identification of bacterial cell division genes and regulators. The method combines the over-expression of a shotgun genomic expression library to perturb the cell division process with high-throughput flow cytometry sorting to screen many thousands of clones. Using this approach, we recovered clones with a filamentous morphology for the model bacterium, Escherichia coli.

 

 

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